In situ hybridization with oligonucleotide probes.
نویسندگان
چکیده
Equipment and reagents • Glass coverslips, 22x22mm • 6-well plates • 4% freshly made paraformaldehyde (Electron Microscopy Sciences) in PBS, pH 7.4 • Triton X-100 (Sigma) • Parafilm • Forceps, Dumont, GG (Electron Microscopy Sciences) • Avidin-DCS-Texas Red or –fluorescein (Vector) • Filter paper • Mounting medium (Molecular Probes) • Microscopy coverslide • Deionized formamide (Ambion) • Yeast tRNA (10mg/ml) (Sigma)
منابع مشابه
A straightforward DOPE (double labeling of oligonucleotide probes)-FISH (fluorescence in situ hybridization) method for simultaneous multicolor detection of six microbial populations.
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ورودعنوان ژورنال:
- International review of neurobiology
دوره 47 شماره
صفحات -
تاریخ انتشار 2002